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Image Search Results
Journal: Scientific Reports
Article Title: Kidney injury molecule-1 inhibits metastasis of renal cell carcinoma
doi: 10.1038/s41598-021-90919-8
Figure Lengend Snippet: Knockdown of human KIM-1 in 786-O cells and expression if murine KIM-1 in Renca RCC cells. ( A ) 786-O cell lines were transduced using lentivirus encoding either KIM-1 shRNA or a control shRNA plasmid. RT-qPCR of 786-O cell lines normalized to housekeeping gene (GAPDH), showing successful knockdown of KIM-1 mRNA in 786-O-shKIM-1, but not in 786-O-shControl (*p = 0.0248). ( B ) Western Blot analyses confirming KIM-1 protein knockdown in 786-O-shKIM-1, but not 786-O-shControl cell lines. ( C ) Renca cell lines were transduced using lentivirus encoding either KIM-1 expressing (KIM-1 pos ) or non-expressing (KIM-1 neg ) vectors. RT-qPCR of Renca cell lines normalized to housekeeping gene (GAPDH), showing successful upregulation of KIM-1 in Renca KIM-1 pos , but not Renca KIM-1 neg cell lines (**p < 0.001). ( D ) Western blot analyses confirming strong cellular KIM-1 protein expression in Renca KIM-1 pos , but not in Renca KIM-1 neg cell lines. The cropped Western blots images in ( A ) and ( B ) were obtained from the same gel but the resulting membranes were cut and probed with either anti-KIM-1 or anti-GAPDH antibodies for ( A ). The exposures was optimized by the digital imaging system.
Article Snippet: Control transduction was done using scrambled
Techniques: Knockdown, Expressing, shRNA, Control, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Imaging